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cervical cancer cell lines caski (ATCC)

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ATCC cervical cancer cell lines caski
Cervical Cancer Cell Lines Caski, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cervical+cancer+cell+line+caski/SiHa/pm42172712-51-2-25
Average 98 stars, based on 2236 article reviews

cervical cancer cell lines caski - by Bioz Stars, 2026-09

98/100 stars

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Cell Culture:

Article Title: Transcriptomic profile induced by calcitriol in CaSki human cervical cancer cell line.
Article Snippet: .. The human cervical cancer cell line CaSki, obtained from the American Type Culture Collection (CRL-1550, Manassas, VA, USA), was cultured in growth medium (RPMI 1640 medium supplemented with 5% heat-inactivated fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin). .. For experimental treatments, cells were seeded and incubated for 24 hours before being exposed to either calcitriol at a concentration of 10 nM (Sigma-Aldrich, St. Louis, MO, USA) or 0.1% ethanol as a vehicle for an additional 24 hours period in treatment medium (RPMI medium supplemented with 5% charcoal-stripped fetal bovine serum).

Article Title: Transcriptomic profile induced by calcitriol in CaSki human cervical cancer cell line
Article Snippet: .. The human cervical cancer cell line CaSki, obtained from the American Type Culture Collection (CRL-1550, Manassas, VA, USA), was cultured in growth medium (RPMI 1640 medium supplemented with 5% heat-inactivated fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin). .. For experimental treatments, cells were seeded and incubated for 24 hours before being exposed to either calcitriol at a concentration of 10 nM (Sigma-Aldrich, St. Louis, MO, USA) or 0.1% ethanol as a vehicle for an additional 24 hours period in treatment medium (RPMI medium supplemented with 5% charcoal-stripped fetal bovine serum).

Article Title: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity.
Article Snippet: A375 and MCF7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) high glucose (Sigma-Aldrich, cat. no. D6429) supplemented with 10% fetal bovine serum (FBS; Life Technologies, cat. no. A3840102) and 1× penicillin–streptomycin (Pen–Strep; Cytiva, cat. no. SV30010). .. The cervical cancer cell line CaSki (ATCC, cat. no. CRL-1550) was cultured in Roswell Park Memorial Institute (RPMI) medium with GlutaMAX (Gibco, cat. no. 72400-047) supplemented with 10% FBS and 1× Pen–Strep. .. The Lenti-X HEK293T cell line was obtained from Takara Bio (cat. no. 632180) and cultured in DMEM high glucose supplemented with 10% FBS.

Article Title: MicroRNA-183-5p negatively regulates interleukin-8 expression in cervical cancer cells
Article Snippet: .. Cervical cancer cell line CaSki was brought from the American type culture collection (ATCC) and the cells were cultured as described by ATCC manufactured instructions. ..

Article Title: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity
Article Snippet: A375 and MCF7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) high glucose (Sigma-Aldrich, cat. no. D6429) supplemented with 10% fetal bovine serum (FBS; Life Technologies, cat. no. A3840102) and 1× penicillin–streptomycin (Pen–Strep; Cytiva, cat. no. SV30010). .. The cervical cancer cell line CaSki (ATCC, cat. no. CRL-1550) was cultured in Roswell Park Memorial Institute (RPMI) medium with GlutaMAX (Gibco, cat. no. 72400-047) supplemented with 10% FBS and 1× Pen–Strep. .. The Lenti-X HEK293T cell line was obtained from Takara Bio (cat. no. 632180) and cultured in DMEM high glucose supplemented with 10% FBS.

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$a , MYC ORF-based overexpression sensitizes cervical cancer cells to T cell cytotoxicity. The fraction of surviving HPV + cervical cancer cells ( y axis) in coculture with E7 TCR T cells (1:1, 48 h), is shown for CaSki cells transduced to express a control, BID or MYC ORF (mean ± s.d., n = 3 technical replicates per ORF). **** P < 0.001, ordinary one-way ANOVA, Dunnett’s multiple-comparison test. b , Fold-change in A375 cell viability after 24-h treatment with FasL (200 ng ml −1 ), shown for A375 cells with ORF-based overexpression of different sensitizing hits compared to A375 cells with a control ORF (mean ± s.d., n = 3–6 technical replicates per ORF). **** P < 0.0001, ** P < 0.01, ordinary one-way ANOVA, Dunnett’s multiple-comparison test, compared to control cells. c , UMAP of Perturb-seq data of control (NTC) and MYC CRISPRa cells, colored based on: (1) culture conditions, (2) sgRNA and MYC expression level, (3) the expression of the MYC GA signature and (4) the expression of the CRISPRa-resistance hit GAS7 (log 2 1p-transformed tp100k). d , Expression of MYC GA signature in control (NTC) cells and cells with MYC CRISPRa sgRNAs, further stratified based on MYC expression in monoculture (left) and coculture (right). The number of cells in each group is shown in parentheses ( n ). Boxplots: the middle line shows the median, the box edges show the 25th and 75th percentiles and the whiskers show the most extreme points that do not exceed ±1.5× the interquartile range (IQR). Further outliers are marked individually with circles (minima or maxima). **** P < 0.0001, one-tailed Student’s t -test.$

Journal: Nature Genetics

Article Title: High-content CRISPR activation screens identify synthetically lethal RNA-based mechanisms to sensitize cancer cells to targeted T cell cytotoxicity

doi: 10.1038/s41588-026-02561-7

Figure Lengend Snippet: a , MYC ORF-based overexpression sensitizes cervical cancer cells to T cell cytotoxicity. The fraction of surviving HPV + cervical cancer cells ( y axis) in coculture with E7 TCR T cells (1:1, 48 h), is shown for CaSki cells transduced to express a control, BID or MYC ORF (mean ± s.d., n = 3 technical replicates per ORF). **** P < 0.001, ordinary one-way ANOVA, Dunnett’s multiple-comparison test. b , Fold-change in A375 cell viability after 24-h treatment with FasL (200 ng ml −1 ), shown for A375 cells with ORF-based overexpression of different sensitizing hits compared to A375 cells with a control ORF (mean ± s.d., n = 3–6 technical replicates per ORF). **** P < 0.0001, ** P < 0.01, ordinary one-way ANOVA, Dunnett’s multiple-comparison test, compared to control cells. c , UMAP of Perturb-seq data of control (NTC) and MYC CRISPRa cells, colored based on: (1) culture conditions, (2) sgRNA and MYC expression level, (3) the expression of the MYC GA signature and (4) the expression of the CRISPRa-resistance hit GAS7 (log 2 1p-transformed tp100k). d , Expression of MYC GA signature in control (NTC) cells and cells with MYC CRISPRa sgRNAs, further stratified based on MYC expression in monoculture (left) and coculture (right). The number of cells in each group is shown in parentheses ( n ). Boxplots: the middle line shows the median, the box edges show the 25th and 75th percentiles and the whiskers show the most extreme points that do not exceed ±1.5× the interquartile range (IQR). Further outliers are marked individually with circles (minima or maxima). **** P < 0.0001, one-tailed Student’s t -test.

Article Snippet: The cervical cancer cell line CaSki (ATCC, cat. no. CRL-1550) was cultured in Roswell Park Memorial Institute (RPMI) medium with GlutaMAX (Gibco, cat. no. 72400-047) supplemented with 10% FBS and 1× Pen–Strep.

Techniques: Over Expression, Control, Comparison, Expressing, Transformation Assay, One-tailed Test